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Isolation and molecular characterization of potential cellulolytic bacillus species isolated from soil samples
Other Title(s)
عزل و تعريف بكتيريا الباسيلس ذات الكفاءة العالية في إنتاج إنزيم السليلولاز من عينات التربة
Joint Authors
Abd al-Aziz, Isra Abd al-Hamid
Mahmud, Ala Rubi
Hassan, Jamal Muhammadayn
al-Dasuqi, Tharwat al-Sayyid
Source
Fayoum Journal of Agricultural Research and Development
Issue
Vol. 34, Issue 2 (31 Jul. 2020), pp.1-12, 12 p.
Publisher
Fayoum University Faculty of Agriculture
Publication Date
2020-07-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Earth Sciences, Water and Environment
Topics
Abstract EN
The twenty-two bacterial isolates were screened for cellulolytic activity using Congo red stain on Carboxymethylcellulose (CMC) agar plates inoculated with the isolates.
All the isolates were found to hydrolyze Carboxymethylcellulose.
Five bacterial isolates (FAY88, FAY103, FAY117.
FAY136 and FAY182) were selected according to their higher production of carboxymethyl cellulase (CMCase).
A Gram stain test was carried out to identify five isolates as Gram-positive rods, all associated mainly with members of the Bacillus.
Their CMCase activities were 1.49, 1.26, 1.21, 1.21 and 1.24 U/ml, respectively.
Genetic distances for the five selected strains with high cellulase activity based on the 79 bands obtained from the results of RAPD revealed the similarity according to the morphological characterization agrees with genetic distance.
The SDS-PAGE analysis was revealed that, the molecular weight of crude cellulase isolated from Bacillus isolates was found approximately of 54 kDa.
American Psychological Association (APA)
Abd al-Aziz, Isra Abd al-Hamid& Hassan, Jamal Muhammadayn& Mahmud, Ala Rubi& al-Dasuqi, Tharwat al-Sayyid. 2020. Isolation and molecular characterization of potential cellulolytic bacillus species isolated from soil samples. Fayoum Journal of Agricultural Research and Development،Vol. 34, no. 2, pp.1-12.
https://search.emarefa.net/detail/BIM-1274659
Modern Language Association (MLA)
Abd al-Aziz, Isra Abd al-Hamid…[et al.]. Isolation and molecular characterization of potential cellulolytic bacillus species isolated from soil samples. Fayoum Journal of Agricultural Research and Development Vol. 34, no. 2 (Jul. 2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1274659
American Medical Association (AMA)
Abd al-Aziz, Isra Abd al-Hamid& Hassan, Jamal Muhammadayn& Mahmud, Ala Rubi& al-Dasuqi, Tharwat al-Sayyid. Isolation and molecular characterization of potential cellulolytic bacillus species isolated from soil samples. Fayoum Journal of Agricultural Research and Development. 2020. Vol. 34, no. 2, pp.1-12.
https://search.emarefa.net/detail/BIM-1274659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 10-11
Record ID
BIM-1274659