Study of genetic differences of carnation cultivars using RAPD indicators

Abstract EN

The experiment was conducted in the Biotechnology Laboratory in the Department of Horticulture and Landscaping, College of Agriculture, Al-Qasim Green University.

The technique of random replication polymorphism of DNA sequence (RAPD) with seven primers was adopted to detect genetic differences between ten carnation cultivars (local, Bizet, Shridea, Orange, Amprose, Mariposa, Grand, White, Viana, Liberty).

The work steps included isolating and purifying the DNA of the newly-grown leaves of the cultivars included in the research and detecting the discrepancies between the replicated pieces of each cultivar after the samples were migrated by the electrophoresis device in the RAPD reactions.

The results of genetic analysis with RAPD indicators showed a clear difference in the number of DNA replication bands and their molecular weights, depending on the primer used.

The total number of differentiated bands reached 368 bundles for all the primers used.

The studied varieties were divided into two main groups A and B, the similarity ratio between the two groups was 350.

, included Group A had eight varieties, while group B included the two cultivars Viana and Liberty, which indicates that the two cultivars are genetically farthest from the rest of the cultivars, and the similarity ratio between the two cultivars was 850.

As for the two plant cultivars, Orange and Mariposa, the highest percentage of similarity between the cultivars was 940.

After calculating the percentage of the genetic dimension between the cultivars, genetic variations were found in them.

The high genetic differences between the carnation cultivars may be due to the geographical locations to which they belong, in addition to the breeding and improvement processes that were conducted on them.