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Validation of half-reaction volumes of the promega PowerPlex® forensic amplification kits (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System) in STR analysis
Other Title(s)
التحقق من فاعلية استخدام نصف حجم التفاعل في مجموعة محاليل تكثير الحمض النووي الجنائي الخاص ب (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System)
Joint Authors
Salih, Khalifah M.
Hasan, Dhurgham H.
Sadiq, Maryam A.
Muhammad, Basmah T.
Muhammad, Maryam K.
Nahi, Sarwa M.
Baqir, Sara S.
Mahmud, Hanan K.
Salman, Nadiyah F.
Source
Arab Journal of Forensic Sciences and Forensic Medicine
Issue
Vol. 2, Issue 2 (31 Dec. 2020), pp.152-158, 7 p.
Publisher
Naif Arab University for Security Sciences Arab Society for Forensic Sciences and Forensic Medicine
Publication Date
2020-12-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Abstract EN
DNA amplification is known to be the most expensive step during forensic DNA analysis.
This study evaluated the half-reaction amplification protocol (12.5 µL PCR product) using DNA amplification kits from Promega PowerPlex® (PowerPlex® 18D System, PowerPlex ®21System, PowerPlex® Fusion System and PowerPlex® Y23 System), which might aid in reducing sample analysis cost by half and allow the analysis of more samples.
A sensitivity study (15 samples) along with testing of various blood stain samples (n=100) that were submitted to the Medico-Legal Directorate laboratory for DNA testing was accomplished to compare the DNA profiles resulting from half-reaction volume procedure to those with full-reaction volume procedure, using three differed methods along with standard protocol to evaluate the effect of half reaction volume with some variables.
Results demonstrated the use of half-reaction amplification protocol preceded by washing step for all aforementioned DNA amplification kits gave a robust and reliable amplification result that aid to increase the number of samples analyzed and decreased the test cost for each kit without compromising the quality of 3DNA profiles obtained.
American Psychological Association (APA)
Mahmud, Hanan K.& Salman, Nadiyah F.& Hasan, Dhurgham H.& Salih, Khalifah M.& Sadiq, Maryam A.& Muhammad, Basmah T.…[et al.]. 2020. Validation of half-reaction volumes of the promega PowerPlex® forensic amplification kits (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System) in STR analysis. Arab Journal of Forensic Sciences and Forensic Medicine،Vol. 2, no. 2, pp.152-158.
https://search.emarefa.net/detail/BIM-1276898
Modern Language Association (MLA)
Mahmud, Hanan K.…[et al.]. Validation of half-reaction volumes of the promega PowerPlex® forensic amplification kits (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System) in STR analysis. Arab Journal of Forensic Sciences and Forensic Medicine Vol. 2, no. 2 (2020), pp.152-158.
https://search.emarefa.net/detail/BIM-1276898
American Medical Association (AMA)
Mahmud, Hanan K.& Salman, Nadiyah F.& Hasan, Dhurgham H.& Salih, Khalifah M.& Sadiq, Maryam A.& Muhammad, Basmah T.…[et al.]. Validation of half-reaction volumes of the promega PowerPlex® forensic amplification kits (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System) in STR analysis. Arab Journal of Forensic Sciences and Forensic Medicine. 2020. Vol. 2, no. 2, pp.152-158.
https://search.emarefa.net/detail/BIM-1276898
Data Type
Journal Articles
Language
English
Record ID
BIM-1276898