The role of immunohistochemical markers in detecting Helicobacter Pylori

Abstract EN

Helicobacter pylori is one of the most common bacterial pathogens that affect humans.

It causes chronic gastritis in its active and inactive forms and has an important role in various gastric disorders .

Gastric endoscopical biopsy is the main diagnostic method for detecting H.

pylori.

This study aims to find the best methods for detecting H.

pylori by using IHC markers and comparing it with the modified Giemsa stain which is routinely used in laboratories.

The study was conducted on 170 gastric biopsies that were taken by upper gastrointestinal endoscopy and received at the department of pathology in Tishreen University Hospital in Lattakia.

Sections of the samples were stained with H&E, modified Giemsa stains, and HP IHCl marker.

The histological study determined the patterns and degrees of gastritis.

H.

pylori-positive cases were identified using Giemsa staining and IHC markers in all forms of gastritis.

The study showed that the number of positive cases detected with IHC was higher by 36% (46 cases more) than the number of cases stained with modified Giemsa.

When assessing these cases, it was found that H.

pylori might present with a few small oval or coccoid forms and it could be scattered in uncommon sites which prevented it from being detected with routine Giemsa stain.

The study concluded the necessity of using IHC markers in the diagnosis of H.

pylori in gastric biopsies that showed histological signs of inflammation with negative H.pylori results when stained with modified Giemsa stain