The role of some tumour associated genes (ca9, wt1, prame)‎ in diagnosis and prognosis of breast cancer

Abstract EN

The aim of the present study is to assess the possible diagnostic and prognostic significance of certain tumour associated genes (CA9, WT1, and PRAME) in relation to tumour size and lymph node status.

In order, the expression of these factors were measuredin the peripheral blood of breast cancer patients (N=55), patients with benign breast lesions (N=10) and apparently healthy controls (N=20).

Quantitative real time polymerase chain reaction (qRT-PCR) was used to assess the expression of the target biomarkers.

In the breast cancer samples 50(91% ) samples were CA9-positive, 8(14.54% ) wereWT1-positive and 5(9.09% ) were PRAME-positive samples.

The expression of CA9-positive was significantly higher in breast cancer sample compared to benign tumour samples and healthy controls.

For lymph node status, the results of all three genes showed that the highest percentage of positive samples 25(50% ), 5(60% ) and 4(80% ) for CA9, WT1 and PRAME genes respectively, were multiple for lymph node status.

The tumour size was significantly associated with the increasedCA9 and WT1 genes expression with tumour size 2.0-2.9 cm, while for PRAME gene the highest percentage of positive samples were with tumour size 1.0-1.9 cm.

This study showed that CA9 gene can be a useful tool for discrimination between malignant and non-malignant breast tumours, the results may also indicatethe diagnostic and prognostic values for this gene.

However, further analysis of a bigger cohort are required to consolidate these initial findings.