Effect of genotype on the response of wheat (Triticum aestivume L.)‎ f1 crosses to anther culture in vitro in the middle of Iraq

Abstract EN

The present research was conducted to evaluate the effect of genotype on the response of seven wheat F1 crosses to anther culture and their ability to initiate callus and haploid green plants (plants with half number of chromosomes) in vitro and the efficiency of chromosome doubling to produce the doubled haploid plants under the conditions predominant in the middle region of Iraq.

Anthers at uninucleate stage were excised and plated in the callus induction medium.

Few weeks later, developed calli were transferred to plant regeneration medium.

Regenerated haploid plants were transferred to the soil in a growth chamber.

Chromosome doubling was applied and doubled haploid plants were produced.

The genotype clearly affected callus induction and green plantlets frequency.

The overall response of F1 crosses grown under environmental conditions predominant in the middle part of Iraq is encouraging, and the pollen-plants could be successfully induced.

The present research was conducted to evaluate the effect of genotype on the response of seven wheat F1 crosses to anther culture and their ability to initiate callus and haploid green plants (plants with half number of chromosomes) in vitro and the efficiency of chromosome doubling to produce the doubled haploid plants under the conditions predominant in the middle region of Iraq.

Anthers at uninucleate stage were excised and plated in the callus induction medium.

Few weeks later, developed calli were transferred to plant regeneration medium.

Regenerated haploid plants were transferred to the soil in a growth chamber.

Chromosome doubling was applied and doubled haploid plants were produced.

The genotype clearly affected callus induction and green plantlets frequency.

The overall response of F1 crosses grown under environmental conditions predominant in the middle part of Iraq is encouraging, and the pollen-plants could be successfully induced.