Evaluation of genetic variability between three lines of chickens based on rapd-pcr and 18s rrna gene sequencing in erbil (iraqi kurdistan region)
Other Title(s)
تقييم التباين الوراثي بين ثلاثة خطوط من الدجاج اعتمادا على Rapd-Pcr و 18S Rrna و التسلسل الجيني في أربيل (إقليم كردستان العراق)
Parallel Title
تقييم التباين الوراثي بين ثلاثة خطوط من الدجاج اعتمادا على Rapd-Pcr و 18S Rrna و التسلسل الجيني في أربيل (إقليم كردستان العراق)
Joint Authors
Khailany, Rozhgar Abd Allah Muhammad
Khdr, Dilger Maghdid
Umarli, Himin Husayn Ali
Ahmad, Aram Muhammad
Salai, Shirku Amin Fatih
Source
Mesopotamia Journal of Agriculture
Issue
Vol. 49, Issue 3 (30 Sep. 2021), pp.12-26, 15 p.
Publisher
University of Mosul College of Agriculture and Forestry
Publication Date
2021-09-30
Country of Publication
Iraq
No. of Pages
15
Main Subjects
Topics
Abstract EN
The current study was carried out to evaluate and identify the genetic variation between Local Black chicken lines with two commercial Layer (Isa Brown) and Broiler (Rose) breeds using RAPD markers and sequencing approach of 18s rRNA gene.
From the result of the RAPD marker, all primers used were produced 152 scorable bands ranging from 2 to 9 with the size ranging from 320 to 2990 bp with percentage polymorphic loci 64.86% among chicken populations.
The highest amplified fragments by primer OPC-11 and lowest by OPAA-03 were 24 and 8, respectively.
The mean of the observed number of alleles (na), effective number of alleles (ne), gene diversity (h), Shannon's information index (I) for all loci found to be 1.6486, 1.5189, 0.2883 and 0.4129, respectively.
The existence of a high level of polymorphisms and targeted (74) loci throughout all chicken populations/primers indicate sufficient genetic distance and more genetic variability among chicken populations using RAPD-PCR techniques.
Result of blasted sequences of 18srRNA gene of local chicken has GenBank accession number MT808178 and MT808179 by BLAST tool against Gallus gallus, it showed the highest identity 95.74% and 94.88% for data of first and second part, respectively.
The overall dendrograms clustered showed that the local chicken was closer to the commercial layer than to the broiler chicken lines.
Therefore, it suggests that improving the local Black chicken line according to the layer breeding program to collect genetically invaluable genetic resources.
American Psychological Association (APA)
Umarli, Himin Husayn Ali& Khdr, Dilger Maghdid& Khailany, Rozhgar Abd Allah Muhammad& Ahmad, Aram Muhammad& Salai, Shirku Amin Fatih. 2021. Evaluation of genetic variability between three lines of chickens based on rapd-pcr and 18s rrna gene sequencing in erbil (iraqi kurdistan region). Mesopotamia Journal of Agriculture،Vol. 49, no. 3, pp.12-26.
https://search.emarefa.net/detail/BIM-1297904
Modern Language Association (MLA)
Umarli, Himin Husayn Ali…[et al.]. Evaluation of genetic variability between three lines of chickens based on rapd-pcr and 18s rrna gene sequencing in erbil (iraqi kurdistan region). Mesopotamia Journal of Agriculture Vol. 49, no. 3 (2021), pp.12-26.
https://search.emarefa.net/detail/BIM-1297904
American Medical Association (AMA)
Umarli, Himin Husayn Ali& Khdr, Dilger Maghdid& Khailany, Rozhgar Abd Allah Muhammad& Ahmad, Aram Muhammad& Salai, Shirku Amin Fatih. Evaluation of genetic variability between three lines of chickens based on rapd-pcr and 18s rrna gene sequencing in erbil (iraqi kurdistan region). Mesopotamia Journal of Agriculture. 2021. Vol. 49, no. 3, pp.12-26.
https://search.emarefa.net/detail/BIM-1297904
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 23-26
Record ID
BIM-1297904