Minimal media strength for in vitro conservation of strawberry (fragaria ananassa)‎ cultures

Abstract EN

An experiment for strawberry Fragaria ananassa cv.

Festival in vitroconservation of cultures for nine months was studied.

Three MS medium strength(full strength MS, ¾ MS, ½ MS, and ¼ MS) and the storage period (3, 6, and 9months) in slowing down or reducing growth.

Results showed a significant effect ofthe medium salt strength, as the full strength treatment was higher by achieved thehighest percentage of viability (survival % ) and storage characteristics of cultures(N% , P% , carbohydrates% , protein % ) and regeneration ability characteristics (shootsand roots number and length).

Three-month storage period achieved the highest ratecompared to the nine-month storage period, which recorded the lowest average forthe studied characteristics.

The full strength treatment for three months storage periodrealized the highest rate compared to treatment ¼ strength for the storage period ofnine months, which recorded the lowest rate of the studied traits.

The application ofminimal strength for in vitro conservation allows strawberry cultures to remain viablewith a slow growth rate and a significant increase in the storage life of the tissues tonine months.