Preparation and characterization of oil nanoemulsion formulations of beauveria bassiana (Bals.-Criv.)‎

Abstract EN

This study aims to prepper stable thermodynamically dilutablenanoemulsion formulation of Beauveria bassiana with the lowest surfactantconcentration that could improve its solubility stability.

Formulations were preparedfrom oil in the water nanoemulsion region of phase diagrams subjected tothermodynamic stability tests.

We found propanetriol was the highest germination rateat 5% and 10% concentration, 46.66 and 53.33% , respectively.

Castor oil achieved a43.00 germination rate at 1% .

Tween 80 gave 54.33 % germination rate at 10% .

WhileTween 20 showed a 48 % germination rate at 5% .

At the concentration, 1% Term 1284gave 43.33% rate germination.

Nanoemulsion composed of propanetriol and nonionicsurfactants, with a mean particle size ranging from 25.08 to 75.35 nm, was formulatedfor various concentrations of the oils and surfactants.

Water in oil emulsion wasprepared using propanetriol oil, Tween 20, Tween 80, Term 1284, and water.

Nanoemulsion of 25.08, 33.75, and 75.35 nm size was obtained at a 45: 15 % ratio ofoil and surfactant, and it was found to be stable.

The larger droplet size 75.35 nm offormulation Tween 20 and the smaller size was 25.08 nm in the formulation of Term1284.

The higher viscosity value was 16 mPas of formulation Tween 80, and thelowest value was 7.80 in the formulation of Term 1284.

To demonstrate the possibleemployment of these systems, they were used to formulate a nanoformulationpesticide.