Genetic variation among certain fish species in terms of evolution and lineages

Abstract EN

Three tests of phylogenetic including likelihood-joining tree, neighbour-joiningtree, and minimum evolution tree have been used based on sox3 gene.

Phylogenetic analysiswas used to detect the genetic affinity and common ancestors for selected species thatbelong to the same or different families.

This study showed the most appropriate methodsfor testing the genetic affinity among species and the methodology of each test according tothe requirement of molecular applications.

Secondary RNA predicted structure andminimum free energy were also included in this study because of their contribution to thedetection of the orthologous gene and variance in RNA folding among species related to thedifferent families.

The genetic distance in the studied populations was calculated to knowthe most appropriate way to find out the genetic similarity among the studied species.

Thelow distance-variance value of each group indicated significant genetic affinity among thespecies of the same family, this result is more consistent with the test of maximumlikelihoodtree indicating the validity of this test to measure the genetic affinity amongspecies that have common ancestors.