Salmonella species isolated from different sources with special reference to biofilm formation

Abstract EN

The aim of this work was to study the distribution of Salmonella serovars among diseased chickens and diarrheic calves, also to determine the genotypic and phenotypic causes of resistance of Salmonella to antimicrobials.

To achieve this aim, two hundred samples (100 diseased chicken samples and 100 fecal swabs from diarrheic calves) were directed to bacteriological examination according to ISO 6579: 2002 A1: 2007.

Then confirmed by biochemical reactions, serotyping and molecular characterization which revealed the isolation of fourteen (14) Salmonella spp.

isolates (9 from calves and 5 from chickens) with a percentage of 9% and 5% respectively.

Also, Antimicrobial susceptibility testing of the total isolates was done which indicated the presence of multidrug resistance among three isolates, one isolate isolated from chickens which was S.

Gallinarum while the other two isolates were S.

Enteritidis and S.

Typhimurium which were isolated from calves.

Biofilm binding activity of the 14 Salmonella isolates showed that two isolates had strong binding activity which was multidrug resistant and 12 isolates showed moderate binding.

With addition of lactic acid 2%, the binding ability had been decreased.