تأثير الحفظ بالتجميد على تحمل الاجهاد المائي و نسبة الانبات للكالس الجنيني لنخيل التمر صنف برحي

Abstract EN

The current study was conducted for the period from october 2014 till July 2017, in order to increase date palm Phoenix dactylifera L water tolerance using plant tissue culture through the treatment of some additives used for freezing.

Date palm embryogenic callus resulting from shoot tip using MS medium was immersed and inoculated in solutions and media for conservation supplemented with sucrose at a concentration of (342.3 g/L) for seven days and at 27 °C in the dark and in liquid nitrogen at -196 C for 6 and 12 week, then the embryo genic callus was grown on medium included PEG concentrations, 0, 5, 10 and 15% .

The effect of the above factors on survival percentage, germination, some of its chemical components including protein, proline, resulted from recent factor complete randomized design was used for data analysis at probability level of 0.05 The results showed that the Ethylene and preservative solutions and concentrations were significant in the percentage of germination.

The interaction coefficient between PEG 5% DMSO and concentration was 10% with the highest rate of 86.7% .

Treatment of PEG for embryogenic callus after 12 weeks of freezing has affected the percentage protein, and the concentration treatment recorded 10% of the highest percentage rate of 22.0.