Extraction and purification of Gallic acid from eucalyptus camaldulensis leaves

Abstract EN

Some parameters for optimizing conditions to gallic acid extraction from Eucalyptus camaldulensis leaves were studied.

Results revealed that maximum yield of gallic acid (GA) was obtained by using 50% ethanol as extraction solvent at 40 Co for 24 hrs .Antioxidant activity of the resulted extract was evaluated against the free radical ABTS, results showed that E.camaldulensis is a good antioxidant source since it gave the same IC50 of the standard antioxidant trolox.

GA was purified from the mentioned extract by 95% ethanol extraction and adsorption chromatography using sephadex LH-20 .HPLC analysis and FTIR spectra confirmed that the purified GA was identical to the standard GA.