Microscopic and molecular diagnosis of mycobacterium tuberculosis in Erbil city-Iraq

Abstract EN

This study was carried out in consultation clinic of the Chest and Respiratory Diseases Center Erbil city-Iraq from 1st January to 31 December 2018 .

The results explained that from total of 1986 consecutive suspects with TB disease in Erbil city 103(%5.2) were pulmonary tuberculosis (PTB) infection depended on microscopic examination of sputum specimens with AFB stain and sputum culture on Lowenstein Jensen Media While histopathology screening (Biopsy- In cooperation with histopathology's physician ) indicate that from 1883 (%94.8) negative cases 8(%0.4) was extrapulmmonary tuberculosis (EPTB) infection.

According to nationality, most cases were Iraqi from Erbil city52 (%50.5) while the number of non-national patients Syrian were 13(%68.4)and others were from different nationalities 6(%31.6), while Iraqi emigrant patients were 32(%31.1).

According to age group most cases were at age group25-34 and 15-24years were 27(%26.2) and 22(%21.3) respectively and only 2(% 1.9) patients were more than 65 years old .Most of patients were male 55(%53.4) and 48 (%46.6) were females.

Detection of (TB) by GeneXpertMTB/RIF assay for contributing to the rapid diagnosis of the disease and their resistance to drug rifampin (RIF) indicated that all 103(%100) samples were positive to GeneXpertMTB the target DNA detected by real time PCR which is needed to start treatment quickly, while in 11(%10.6) samples the Rifampicin resistance DNA was detected which is need treatment with second-line agents.

Conclusion: The results of current study indicate that diagnosis and detection of TB infection by microscope, culture and rapid diagnosis by PCR have the same accuracy.