Anti-fungal activity of lectin isolated from whole body of fasciola gigantic (Cobbold, 1856)‎

Abstract EN

Lectin with molecular weight of 71 Kilo dalton was isolated from whole body of adult worm of Fasciola gigantica parasite and purification by using sephadex G-200.

This lectin is protein in nature (3.6 gm.

/ ml and 0.329 gm.

/ ml before and after purification, respectively), agglutinate human erythrocytes (1: 5120 and 1: 1280 before and after purification, respectively) and T lymphocytes mitogen by using one week age of chicken as model.

Antifungal activity of this material was tested by using four pathogenic fungi (Alternaria solani, Aspergillus flavus, Fusarium globosum and Penicillium exsporum) and by using three methods (pouring one milliliter on PDA, method of holes and the mixing method).

From the results of present study show the better inhibition rat of lectin against fungi were founded in F.

globosum (57.8%, 55.6% and 41.1%, respectively in three methods) and the lower rate of inhibition was founded in A.

solani in mixing method.

The results of current study will provide ability of this worm to use the lectin to defense against any threat.