Using single molecule microscopy technique to achieve elongated time-period blinking using total internal reflection technique
Author
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 24, Issue 7 (31 Dec. 2016), pp.1858-1866, 9 p.
Publisher
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Engineering & Technology Sciences (Multidisciplinary)
Abstract EN
Recent advances in field of optical microscopy have made it possible to detect image and obtain spectroscopic data from individual molecules, revealing information about population distributions (both static and dynamic) as well as photophysical information.
As higher levels of time resolution become available layers of dynamic information are uncovered, elucidating the fine detail of chemical reaction normally hidden within the ensemble.
Single molecule (SM) techniques are especially relevant to macromolecular biochemistry as chemical processes occurring within large molecules are often too complicated to study in the ensemble.
Single Molecule Fluorescence Microscopy (SMFM) is achieved using coherent laser light to excite the fluorophore to a singlet excited state, from which it can relax rapidly.
However this can be followed by either absorption of additional photons, eventually lead to an ionised or “photo-bleached” state, or spin-inversion and non-radiative intersystem crossing to a triplet state.
The excitation volume has been reduced to achieve elongated time-period blinking using Total Internal Reflection.
American Psychological Association (APA)
Muhammad, Asma Hadi. 2016. Using single molecule microscopy technique to achieve elongated time-period blinking using total internal reflection technique. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 24, no. 7, pp.1858-1866.
https://search.emarefa.net/detail/BIM-1315126
Modern Language Association (MLA)
Muhammad, Asma Hadi. Using single molecule microscopy technique to achieve elongated time-period blinking using total internal reflection technique. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 24, no. 7 (2016), pp.1858-1866.
https://search.emarefa.net/detail/BIM-1315126
American Medical Association (AMA)
Muhammad, Asma Hadi. Using single molecule microscopy technique to achieve elongated time-period blinking using total internal reflection technique. Journal of Babylon University : Journal of Applied and Pure Sciences. 2016. Vol. 24, no. 7, pp.1858-1866.
https://search.emarefa.net/detail/BIM-1315126
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1866
Record ID
BIM-1315126