التعريف الشكلي و الجزيئي لفطر العفن الرمادي Botrytis cinerea المعزول محليا من ثمار الفريز

Abstract EN

Botrytis cinerea is an economically important plant pathogen that infects more than 200 plant species in the field, greenhouses and storage.

Some Srawberry fruits infected by gray mold were collected from local markets in Damascus in April 2015 for isolation and identification Botrytis cinerea.

The Sudy was carried out in the period from the date of sample collection in April 2015 to June 2016 in the laboratories of the department of plant biology in the Faculty of Science at the University of Damascus (syria).

The pathogen was isolated on PDA, and morphologically identified basing on the colony morphology, conidiophore shape, conidia size and sclerotia formation.

The results showed that the fungal growth on PDA was in many patterns.

The conidiophores were more or less straight, septate and branched at the apex.

Sizes of the conidia were 8.2 - 10 X 9 - 15 ^m.

The sclerotia were observed on 30 days old colonies as ring along the edge of the Petri dish.

Pathogen taxonomy was further confirmed by PCR using two specific primers, C729+/C729-.

Results indicated that the isolates were Botrytis cinerea as they produce bands of 700 bp.

The closet phylogenetic neighbor according to this DNA fragment sequence data was Botrytis cinerea with 99% of similarity.

Keywords: Botrytis cinerea, Gray mold, Morphology, Molecular identification.