DNA isolation from formalin-fixed paraffin-embedded tissue for the detection of k-rasmutations in pancreatic cancer
Other Title(s)
عزل جزيئات الحامض النووي من الانسجة المثبتة والمطمورة في شمع البارفينللاشخاص المصابين بسرطان البنكرياس للكشف عن الطفرة الجينية k-ras
Author
Source
Issue
Vol. 16, Issue 1 العلوم الصرفة و الهندسية (30 Jun. 2013), pp.1-5, 5 p.
Publisher
Publication Date
2013-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
This study conduct toassess the detection of K-ras gene mutation at codon 12, to estimate the percentage of K-ras gene mutation and pancreatic cancer.
A total of 30 paraffin blocks containing pancreatic cancer tissue were obtained from archive of central laboratory in Duhok government, 50-µm sections was cut from each paraffin-embedded tissues block for DNA extraction and then DNA samples were quantities by spectrophotometry.
K-ras exons 12 was amplified by Real time-PCR from 50 µg of DNA using detection of K-ras gene mutation (QIAGENE, Germany) according to manufactures protocol.
It was found that 19 patients (63.3 %) showed positivity for K-ras mutation test and 11 patients (36.7%) showed negativity with K-ras mutation.
In conclusion, the method that demonstrated in this report provides a simple and fast way to identify K-ras mutation for the purpose of clinical evaluation in human pancreatic cancer.
Further studies concerning oncogenes, including rasgenes, are warranted to elucidate molecular pathogenic mechanisms of Pancreatic cancer.
American Psychological Association (APA)
Ahmad, Ghazwan F.. 2013. DNA isolation from formalin-fixed paraffin-embedded tissue for the detection of k-rasmutations in pancreatic cancer. Journal of Dohuk University،Vol. 16, no. 1 العلوم الصرفة و الهندسية, pp.1-5.
https://search.emarefa.net/detail/BIM-1322738
Modern Language Association (MLA)
Ahmad, Ghazwan F.. DNA isolation from formalin-fixed paraffin-embedded tissue for the detection of k-rasmutations in pancreatic cancer. Journal of Dohuk University Vol. 16, no. 1 Pure and Engineering Sciences (Jun. 2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1322738
American Medical Association (AMA)
Ahmad, Ghazwan F.. DNA isolation from formalin-fixed paraffin-embedded tissue for the detection of k-rasmutations in pancreatic cancer. Journal of Dohuk University. 2013. Vol. 16, no. 1 العلوم الصرفة و الهندسية, pp.1-5.
https://search.emarefa.net/detail/BIM-1322738
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 3-4
Record ID
BIM-1322738