DNA isolation from formalin-fixed paraffin-embedded tissue for the detection of k-rasmutations in pancreatic cancer

Abstract EN

This study conduct toassess the detection of K-ras gene mutation at codon 12, to estimate the percentage of K-ras gene mutation and pancreatic cancer.

A total of 30 paraffin blocks containing pancreatic cancer tissue were obtained from archive of central laboratory in Duhok government, 50-µm sections was cut from each paraffin-embedded tissues block for DNA extraction and then DNA samples were quantities by spectrophotometry.

K-ras exons 12 was amplified by Real time-PCR from 50 µg of DNA using detection of K-ras gene mutation (QIAGENE, Germany) according to manufactures protocol.

It was found that 19 patients (63.3 %) showed positivity for K-ras mutation test and 11 patients (36.7%) showed negativity with K-ras mutation.

In conclusion, the method that demonstrated in this report provides a simple and fast way to identify K-ras mutation for the purpose of clinical evaluation in human pancreatic cancer.

Further studies concerning oncogenes, including rasgenes, are warranted to elucidate molecular pathogenic mechanisms of Pancreatic cancer.