The role of epidermal growth factor on the proliferation and multiplication of albino mice dermal fibroblast cells in vitro

Abstract EN

Dermal fibroblast cells are fast growing cells and can be rapidly expanded in culture.

These cells are suitable for behavioral, functional, biochemical and genomic studies.

So in this study we investigate the mitogenic effect of epidermal growth factor (EGF) on the proliferation and multiplication of mice newborn dermal fibroblast cells in vitro.

Dermal cells were isolated from newborn albino mice (Mus musculus) and were divided into two groups: first group treated with DMEM+10ng EGF/ ml, while the second group treated with DMEM only as control group.

Fibroblast cells are spindle-like shape with a prominent nucleus with unipolar or bipolar and showed high rate of growth in culture.

Results of this study showed that the effect of EGF as a mitogenic factor were increased with the time of administration, so the number of fibroblast cells were highly increased after two weeks from treatment with EGF and these cells tend to form several layers of fibroblast cells in culture.

In conclusion, EGF has an important role in the proliferation and multiplication of newborn dermal fibroblast cells in vitro