The role of epidermal growth factor on the proliferation and multiplication of albino mice dermal fibroblast cells in vitro
Other Title(s)
دور عامل نمو البشرة على توالد و تضاعف خلايا الارومات الليفية الجلدية للفئران البرصاء خارج الجسم الحي
Author
Source
Issue
Vol. 16, Issue 1 العلوم الصرفة و الهندسية (30 Jun. 2013), pp.95-103, 9 p.
Publisher
Publication Date
2013-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Dermal fibroblast cells are fast growing cells and can be rapidly expanded in culture.
These cells are suitable for behavioral, functional, biochemical and genomic studies.
So in this study we investigate the mitogenic effect of epidermal growth factor (EGF) on the proliferation and multiplication of mice newborn dermal fibroblast cells in vitro.
Dermal cells were isolated from newborn albino mice (Mus musculus) and were divided into two groups: first group treated with DMEM+10ng EGF/ ml, while the second group treated with DMEM only as control group.
Fibroblast cells are spindle-like shape with a prominent nucleus with unipolar or bipolar and showed high rate of growth in culture.
Results of this study showed that the effect of EGF as a mitogenic factor were increased with the time of administration, so the number of fibroblast cells were highly increased after two weeks from treatment with EGF and these cells tend to form several layers of fibroblast cells in culture.
In conclusion, EGF has an important role in the proliferation and multiplication of newborn dermal fibroblast cells in vitro
American Psychological Association (APA)
Amin, Avin Abd Allah Muhammad. 2013. The role of epidermal growth factor on the proliferation and multiplication of albino mice dermal fibroblast cells in vitro. Journal of Dohuk University،Vol. 16, no. 1 العلوم الصرفة و الهندسية, pp.95-103.
https://search.emarefa.net/detail/BIM-1322748
Modern Language Association (MLA)
Amin, Avin Abd Allah Muhammad. The role of epidermal growth factor on the proliferation and multiplication of albino mice dermal fibroblast cells in vitro. Journal of Dohuk University Vol. 16, no. 1 Pure and Engineering Sciences (Jun. 2013), pp.95-103.
https://search.emarefa.net/detail/BIM-1322748
American Medical Association (AMA)
Amin, Avin Abd Allah Muhammad. The role of epidermal growth factor on the proliferation and multiplication of albino mice dermal fibroblast cells in vitro. Journal of Dohuk University. 2013. Vol. 16, no. 1 العلوم الصرفة و الهندسية, pp.95-103.
https://search.emarefa.net/detail/BIM-1322748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 100-101
Record ID
BIM-1322748