Effect date of explant taking and plant growth regulators on micropropagation of cestrum nocturnum l.

Abstract EN

This study conducted in laboratory tissue culture to propagation Cestrum nocturnum L.

form vegetative shoots including shoot tips, node segments and Floral buds at 5 stages newly setted bud partly swollen bud, totally swollen and non flushed buds and initially flushed buds, which were excised and culture on MS medium in the experience of the dates.

The best date to take explant was in August and April, where explants were able to survive after treatment with disinfectant.

The highest percentage of response of plantlets from shoot tips and nodal segments were at 82.33% and 95% respectively in August.

The highest number of proliferation 2 shoot from shoot tips and 2.67 shoots from nodal segments in the April and the longest of proliferation of shoots from shoot tips and shoot from nodal segments in April and August (2.23-2.03) and (2.50- 2.40) cm respectively.

While The floral bud collected on various date (day) on different growth stage and culture on MS medium containing 0.01 mgl-1 IBA and 250 mgl-1 L-Glutamine for two weeks The highest percentage of response of plantlets from Partly swollen at 60% and Responded bud and the nature of response 85% developed into flower from Initially flushed The plantlet taken to completion the next stage and the subsequent culture on MS medium supplemented with different concentration of BA at (0, 0.5, 1, 1.5 and 2) mgl -1 combine with (0, 0.5, 1, 1.5 and 2)mgl-1 Kin using node segments and shoot tips as explant.

BA at 0.5mgl-1 combine with 2 mgl-1 Kin produce the highest number of proliferation shoots as 3.5 from node segments and 3 from shoot tips when cultured on MS medium containing 1 mgl-1 BA+1.5 mgl-1 Kin and in the same medium the explant gave the highest number of leaves 6.50 leaves/ node segment and 5.10 leaves/ shoot tips.

Elongated shoots of Cestrum were rooted on half strength MS medium supplemented with NAA and IAA at (0, 0.2, 0.5 and 1) mgl-1 alone.

NAA and IAA at low concentration (0.5 mgl-1) increased the number of developed roots, and the root length (5.8 root/ shoot and the root length 7.5 cm) and (3.7 root/ shoot and the root length 4.7 cm) receptivity.

The plantlets of Cestrum were transferred from rooting medium and cultured in pots containing peat moss and sand (1:1 by volume) under greenhouse conditions.

The plants grow normally and the survival percentage reached 100%.