Prevention of necrotic enteritis in broiler chickens by prebiotics and probiotics VS control by antibiotics : in vivo study

Abstract EN

Clostridium perfringens (C.perfringens) induced necrotic enteritis (NE) has become a worldwide significant problem in broilers industry.

A total of 100 intestinal samples were collected from broilers chickens that demonstrated clinical manifestations and post mortem lesions of Necrotic Enteritis.

Bacteriological examination of samples revealed that C.

perfringens was isolated from (62/100) 62%.

PCR was used to screen the circulating toxigenic subtypes and revealed that 14/20 (70%) of isolates were positive to cpa gene thus belonged to C.

perfringens type A.

Phenotypic antimicrobial resistance patterns was demonstrated by isolates to lincomycin 51/62(82.26%), ampicillin 45/62 (72.6%), nalidixic acid 43/62(69.35%), and spectinomycin 43/62 (69.35%), respectively.

Futhermore, 49/62 (79%) of isolates demonstrated multidrug resistance phenotypes.

Experimental study demonstrated better performance indicators in C.perfringens challenged chicks fed on probiotic (Bacillus subtilis) and prebiotic (Mannan Oligosaccharide / MOS) compared to the challenged chicks treated with amoxicillin.