Effect of pH, sucrose concentrations and medium states on in vitro root-ing of pineapple (Ananas comosus (L) Merr) cv queen
Other Title(s)
تأثير درجة الحموضة و تركيز السكروز و حالة الوسط على التجذير المخبري للأناناس صنف كوين
Author
Source
Al-Mukhtar Journal of Sciences
Issue
Vol. 36, Issue 2 (30 Jun. 2021), pp.135-147, 13 p.
Publisher
Publication Date
2021-06-30
Country of Publication
Libya
No. of Pages
13
Main Subjects
Abstract EN
The effect of medium states (liquid, semi solid, solid), pH (5.0, 5.5, 6.0, 6.5) and sucrose concentrations (10, 20, 30, 40 g/l) on in vitro rooting of pineapple cultured in full strength MS enriched with IBA at 0.5 mg/l were investigated.
According to average overall sucrose concentrations, overall pH adjustments, and at each combination of equal sucrose and pH, liquid medium was always super than solid and semisolid.
The tallest plantlets (66 to 71 mm) obtained in liquid medium enriched with sucrose at 10 and 20 g/l both adjusted to pH 6.0; sucrose at 20 g/l and adjusted to pH 6.5 and sucrose at 30 g/l and adjusted to pH 5.0.
All of the above combinations except sucrose at 20 and pH 6.0 resulted in 100% rooting.
Sucrose at 30 g/l and pH 5.0 resulted in two times more (11 roots per shoot) and three times longer roots (39 mm) than the other treatments (5 roots each about 14 mm long).
Each rooting parameter had different optimum combinations of medium state, sucrose and pH adjustment.
For any parameter, proper pH adjustment could reduce the optimal sucrose enrichment from 30 to 20 and even to 10 g/l.
Hence, pH adjustment is suggested as an important approach for reduction of in vitro rooting medium cost.
American Psychological Association (APA)
Hamad, Abd al-Hamid Mukhtar. 2021. Effect of pH, sucrose concentrations and medium states on in vitro root-ing of pineapple (Ananas comosus (L) Merr) cv queen. Al-Mukhtar Journal of Sciences،Vol. 36, no. 2, pp.135-147.
https://search.emarefa.net/detail/BIM-1324463
Modern Language Association (MLA)
Hamad, Abd al-Hamid Mukhtar. Effect of pH, sucrose concentrations and medium states on in vitro root-ing of pineapple (Ananas comosus (L) Merr) cv queen. Al-Mukhtar Journal of Sciences Vol. 36, no. 2 (2021), pp.135-147.
https://search.emarefa.net/detail/BIM-1324463
American Medical Association (AMA)
Hamad, Abd al-Hamid Mukhtar. Effect of pH, sucrose concentrations and medium states on in vitro root-ing of pineapple (Ananas comosus (L) Merr) cv queen. Al-Mukhtar Journal of Sciences. 2021. Vol. 36, no. 2, pp.135-147.
https://search.emarefa.net/detail/BIM-1324463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 144-146
Record ID
BIM-1324463