Synthesis and characterization of new mannich base and study of their binding with CT-DNA

Abstract EN

The reaction is divided into two main parts.

The first part involves the synthesized compounds derived from Indole.

The second part involves binding some synthesized compounds with Calf Thymus DNA.

To clarify the work more broadly-The first type a series of Mannich bases for Indole were synthesized by react of Indole with( morpholine, diethylamine and Proline) in the presence of acetaldehyde for synthesized of (A1 ,A2 ,A3 ) after that react of (A1 ,A2 ,A3 ) with Cytosine in presence of acetaldehyde for synthesized of(A4 ,A5 ,A6 ).

All reactions were followed by using Thin Layer chromatography, the derivatives have been purified by column Chromatography and characterized on the basis of IR and N.M.R .The second part was selecting mannich derivatives and study interaction with CT-DNA by studying the effect of binding on CT-DNA structure, by using FT-IR and UV-Visible techniques.