Evaluation of molecular technique and microscopical examination for diagnosis of blood parasites in equine and camels
Joint Authors
Mahmud, Abd al-Karim A.
Abd al-Rahman, Amir H.
Hijazi, Imad N.
Khidr, Adil M.
al-Shimi, Tharwat M.
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 55, Issue 1 (31 Oct. 2017), pp.217-224, 8 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2017-10-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study was designed to evaluate PCR technique as a diagnostic tool for blood parasites infection in equine and camels in relation to conventional microscopic examination of stained blood smear.
150 equine stained blood smears were examined microscopically, 15 of them were suspected to be infested with equine piroplasmosis.
10 samples (including one positive by blood film) were examined by conventional PCR technique, 7 samples were positive.
135 camels stained blood smears were examined microscopically, 2 of them were infested with trypanosoma, and 10, 5 and 15 samples were suspected to be infested with theileria spp., babesia spp.
and anaplasma spp., respectively.
10 samples (including 2 positive for trypanosoma), 10 negative for theileria, 10 negative for babesia and 10 negative for anaplasma by blood film were examined separately by conventional PCR technique, 9, 1, 1 and 5 samples were positive, respectively.
The results concluded that PCR assays are more sensitive than blood film examination.
American Psychological Association (APA)
Hijazi, Imad N.& Mahmud, Abd al-Karim A.& Khidr, Adil M.& al-Shimi, Tharwat M.& Abd al-Rahman, Amir H.. 2017. Evaluation of molecular technique and microscopical examination for diagnosis of blood parasites in equine and camels. Alexandria Journal of Veterinary Sciences،Vol. 55, no. 1, pp.217-224.
https://search.emarefa.net/detail/BIM-1327659
Modern Language Association (MLA)
Hijazi, Imad N.…[et al.]. Evaluation of molecular technique and microscopical examination for diagnosis of blood parasites in equine and camels. Alexandria Journal of Veterinary Sciences Vol. 55, no. 1 (Oct. 2017), pp.217-224.
https://search.emarefa.net/detail/BIM-1327659
American Medical Association (AMA)
Hijazi, Imad N.& Mahmud, Abd al-Karim A.& Khidr, Adil M.& al-Shimi, Tharwat M.& Abd al-Rahman, Amir H.. Evaluation of molecular technique and microscopical examination for diagnosis of blood parasites in equine and camels. Alexandria Journal of Veterinary Sciences. 2017. Vol. 55, no. 1, pp.217-224.
https://search.emarefa.net/detail/BIM-1327659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 223-224
Record ID
BIM-1327659