Biochemical and molecular characterization of aeromonas species isolated from fish

Abstract EN

In this study, a total of 150 Oreochromis niloticus (Nile tilapia) (90) and Ictalurus punctatus (Channel cat fish) (60) fish were collected randomly during an outbreak of disease mass mortalities from different fish farms in El Sharkia and El Ismailia governorate.

Clinical signs and postmortem were recorded.

Aeromonas veronii were isolated and presumptively identified using API20E system with recovery rate of 36.66% from total number of fish.

The identification was confirmed for Aeromonas veronii biovar sobria by PCR and sequence analysis of 16s rRNA gene.

The experimental infection was carried out by Aeromonas veronii biovar sobria isolate with 60 % of mortality rate.

By antibiotic sensitivity test, Aeromonas veronii biovar sobria isolate was sensitive to Trimethoprim + Sulphamethoxazole and Erythromycin but it was resistant to Oxytetracyclin.