Detection the difference in growth of equine herpesvirus-1 and 4 in vitro by using microscopic examinations and Flowcytometric analysis
Author
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 52, Issue 1 (31 Jan. 2017), pp.97-102, 6 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2017-01-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Equine herpesvirus - 1 (EHV-1) and Equine herpesvirus - 4 (EHV-4) are endemic in horse over the entire world, in the study we compared between the replication of EHV-1 and 4 in an equine-derived cell line (FHK-Tcl3.1) by using microscopic examination and flowcytometric analysis.
The result of EHV-1 showed that the CPE effect in the FHK-Tcl3.1 cells appeared after 24 hr from infection, but in case of EHV-4 cytopathic effect (CPE) appeared after 48 hr from infection.
The flowcytometric analysis result for detection the percent of infected cells by both viruses, were (19.7 %) after four days from infection in EHV-4, compared to EHV-1 (50.3%), which the cells harvest in the second day from infection.
These data suggested that the difference in cellular entry might be playing the decisive role in determining pathogenicity of EHV- 1 and EHV-4.
American Psychological Association (APA)
Mahmud, Hasan Y. A. H.. 2017. Detection the difference in growth of equine herpesvirus-1 and 4 in vitro by using microscopic examinations and Flowcytometric analysis. Alexandria Journal of Veterinary Sciences،Vol. 52, no. 1, pp.97-102.
https://search.emarefa.net/detail/BIM-1328219
Modern Language Association (MLA)
Mahmud, Hasan Y. A. H.. Detection the difference in growth of equine herpesvirus-1 and 4 in vitro by using microscopic examinations and Flowcytometric analysis. Alexandria Journal of Veterinary Sciences Vol. 52, no. 1 (Jan. 2017), pp.97-102.
https://search.emarefa.net/detail/BIM-1328219
American Medical Association (AMA)
Mahmud, Hasan Y. A. H.. Detection the difference in growth of equine herpesvirus-1 and 4 in vitro by using microscopic examinations and Flowcytometric analysis. Alexandria Journal of Veterinary Sciences. 2017. Vol. 52, no. 1, pp.97-102.
https://search.emarefa.net/detail/BIM-1328219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 101-102
Record ID
BIM-1328219