Prevalence and improvement of culture independent quantification of clostridium perfringens in broiler's intestine
Joint Authors
Sami, Ahmad
Nasif, Suad A.
Abd al-Ati, Mayy F.
Abd al-Wahhab, Ashraf M.
Amir, Jamal A.
Ammar, Ahmad M.
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 52, Issue 1 (31 Jan. 2017), pp.160-165, 6 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2017-01-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Clostridium Perfringens represent one of the natural inhabitant bacteria in the gastrointestinal tract of chickens.
However, predisposing factors, mainly co-infection with Coccidia, represent a favorable condition for over growth of C.
perfringens that induce necrotic enteritis (NE).
The prevalence of C.
perfringens in commercial broilers with enteric disease in Egypt was investigated via bacteriological isolation and identification and then confirmed by toxin typing by PCR.
Furthermore, culture-independent quantitative real time PCR targeting alpha toxin gene was improved via reducing the effect of PCR inhibitors in the intestinal content (IC).
Out of 61 broiler flocks, 40 were positive for C.
perfringens with a higher incidence in winter (81.25%) and in 20-30 days old broilers (66.67%).
All isolates belonged to C.
perfringens type A with the absence of enterotoxin or Beta2 toxin genes.
Pretreatment of the intestinal content with acetone improve the detection limit of quantitative real time PCR assay to 102 cells/gm which was highly correlated to the culture-based counting method using cooked meet media.
C.
perfringes type A widespread in broilers in Egypt and the extraction method used herein could be used for direct quantification of C.
perfringens in the intestinal content of broiler chickens without prior enrichment.
American Psychological Association (APA)
Sami, Ahmad& Nasif, Suad A.& Abd al-Ati, Mayy F.& Abd al-Wahhab, Ashraf M.& Amir, Jamal A.& Ammar, Ahmad M.. 2017. Prevalence and improvement of culture independent quantification of clostridium perfringens in broiler's intestine. Alexandria Journal of Veterinary Sciences،Vol. 52, no. 1, pp.160-165.
https://search.emarefa.net/detail/BIM-1328225
Modern Language Association (MLA)
Sami, Ahmad…[et al.]. Prevalence and improvement of culture independent quantification of clostridium perfringens in broiler's intestine. Alexandria Journal of Veterinary Sciences Vol. 52, no. 1 (Jan. 2017), pp.160-165.
https://search.emarefa.net/detail/BIM-1328225
American Medical Association (AMA)
Sami, Ahmad& Nasif, Suad A.& Abd al-Ati, Mayy F.& Abd al-Wahhab, Ashraf M.& Amir, Jamal A.& Ammar, Ahmad M.. Prevalence and improvement of culture independent quantification of clostridium perfringens in broiler's intestine. Alexandria Journal of Veterinary Sciences. 2017. Vol. 52, no. 1, pp.160-165.
https://search.emarefa.net/detail/BIM-1328225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 164-165
Record ID
BIM-1328225