A trial for vaccination of sheep against caseous lymphadentitis using oil adjuvent bacterin enhanced by bacillus calmette-guerin vaccine

Abstract EN

Corynebacterium pseudotuberculosis is the etiologic agent of Caseous lymphadenitis, A disease that affects goats and sheep and can cause severe economic loss, Characterized by abscess development in subcutaneous tissues, Lymph nodes and internal organs.In this study we prepare a formalized vaccine from local strain combined with montanide oil adjuvant.

The prepared vaccine was assessed in two groups of local balady bread sheep in comparison to a negative control group (5 animals each).

Group I vaccinated with vaccine+BCG and group II vaccinated with the vaccine only.

Both were in the same age, same sex and free from CLA lesions.

Both had a booster dose at 21 day post vaccination.

The samples were collected at days 0, 10, 21, 44, and 60 post vaccination.

The cellular immune response was evaluated by phagocytic activity and index, and differential leukocytes count and the humeral immune response was evaluated by ELISA (Enzyme- linked immunosorbent assay) test.

The result reveal a significant increase (p<0.05) in cellular and humeral immunity respond and prepared vaccine with BCG (Bacillus Calmette–Guerin vaccine) could be used for prevention and control of CLA (Caseous lymphadenitis) in sheep.