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Improvement of in vitro fertilization in buffalo by increasing the fertilizing capacity of spermatozoa
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 53, Issue 2 (30 Apr. 2017), pp.6-10, 5 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
In vitro fertilization (IVF) is a necessary procedure in the in vitro production of embryo.
spermatozoa need to undergo capacitation to be able to perform the acrosome reaction which is required for zona pellucida penetration.
different additives are used to aid in that process, such as caffeine and theophylline.
In this study, the effects of IVF media supplementation with d-penicillamine, hypotaurine and epinephrine (PHE) on fertilization and cleavage rates were assessed.
Cumulus oocyte complexes (COCs) containing a compact cumulus cells and normal homogenous cytoplasm were selected.
in vitro maturation (IVM) was performed in TCM199 supplied with hormones overnight in a culture conditions of 38.5°C and 5% CO2 in air.
After 20-22 hr.
of maturation, oocytes were randomly assigned to five groups of sperm-TALP media (control and four different PHE concentrations) then inseminated.
the fertilization rate of the expected embryos were assessed after 22 hr.
after staining with aceto-orcin 1% or cultured for 48 hr.
to estimate the cleavage rate.
the current data revealed that, 25 μl/ml PHE significantly (P< 0.05) improved fertilization (55.93 vs 36.96%) and cleavage (46.15 vs 22.22%) rates when compared to control.
American Psychological Association (APA)
al-Rubi, Ahmad M.& Husayn, Mamduh S.& Budayr, Wail& Muntasir, Abd al-Munim M.& Badr, Majdi Ramadan& Hijab, Abd al-Rauf O.…[et al.]. 2017. Improvement of in vitro fertilization in buffalo by increasing the fertilizing capacity of spermatozoa. Alexandria Journal of Veterinary Sciences،Vol. 53, no. 2, pp.6-10.
https://search.emarefa.net/detail/BIM-1332520
Modern Language Association (MLA)
al-Rubi, Ahmad M.…[et al.]. Improvement of in vitro fertilization in buffalo by increasing the fertilizing capacity of spermatozoa. Alexandria Journal of Veterinary Sciences Vol. 53, no. 2 (Apr. 2017), pp.6-10.
https://search.emarefa.net/detail/BIM-1332520
American Medical Association (AMA)
al-Rubi, Ahmad M.& Husayn, Mamduh S.& Budayr, Wail& Muntasir, Abd al-Munim M.& Badr, Majdi Ramadan& Hijab, Abd al-Rauf O.…[et al.]. Improvement of in vitro fertilization in buffalo by increasing the fertilizing capacity of spermatozoa. Alexandria Journal of Veterinary Sciences. 2017. Vol. 53, no. 2, pp.6-10.
https://search.emarefa.net/detail/BIM-1332520
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-10
Record ID
BIM-1332520