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Isolation and improvement of some proteolytic enzymes (lipase, amylase and protease) produced from local Aspergillus sp.
Joint Authors
Abd al-Raziq, A. B.
Mustafa, Mayy M.
al-Nadi, Ghadah H.
Ibrahim, S. A.
Source
Arab Journal of Agriculture Sciences
Issue
Vol. 5, Issue 14 (30 Apr. 2022), pp.85-105, 21 p.
Publisher
The Arab Institution for Education Science and Art
Publication Date
2022-04-30
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Topics
Abstract EN
Fourteen fungal isolates were isolated from soil, ten were found as aspergillus Niger and four aspergillus terreus.
three enzymes (lipase, amylase and protease) were determined qualitatively and quantitively by chemical methods.
tow isolates from a.
Niger and A.
terreus highly productive was genetically modified by ultraviolet (UV).
from each isolate, 50 mutants were randomized obtained and qualitatively tested.
ten of them were quantitatively determined.
different response was obtained for the three enzymes.
after UV irradiation lipase was the most producer, then protease showed higher production in A.
terreus only, while production decreased in a.
Niger, while amylase it showed a slight difference in both isolates compared to the wild.
genetic relationships of the mutants of A.
niger and A.
terreus were analyzed with a randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR).
results obtained 61.22% genetic similarity (49 total bands) was calculated between parental and mutant strain of A.
niger and 48.88% genetic similarity (45 total bands) for A.
terrues.
American Psychological Association (APA)
Mustafa, Mayy M.& al-Nadi, Ghadah H.& Abd al-Raziq, A. B.& Ibrahim, S. A.. 2022. Isolation and improvement of some proteolytic enzymes (lipase, amylase and protease) produced from local Aspergillus sp.. Arab Journal of Agriculture Sciences،Vol. 5, no. 14, pp.85-105.
https://search.emarefa.net/detail/BIM-1333110
Modern Language Association (MLA)
Mustafa, Mayy M.…[et al.]. Isolation and improvement of some proteolytic enzymes (lipase, amylase and protease) produced from local Aspergillus sp.. Arab Journal of Agriculture Sciences Vol. 5, no. 14 (Apr. 2022), pp.85-105.
https://search.emarefa.net/detail/BIM-1333110
American Medical Association (AMA)
Mustafa, Mayy M.& al-Nadi, Ghadah H.& Abd al-Raziq, A. B.& Ibrahim, S. A.. Isolation and improvement of some proteolytic enzymes (lipase, amylase and protease) produced from local Aspergillus sp.. Arab Journal of Agriculture Sciences. 2022. Vol. 5, no. 14, pp.85-105.
https://search.emarefa.net/detail/BIM-1333110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references: p. 102-105
Record ID
BIM-1333110