Genetic diversity among Spilocaea oleagina isolates from different regions in Palestine
Other Title(s)
التنوع الوراثي بين عزلات فطر Spilocaea oleagina من مناطق مختلفة في فلسطين
Joint Authors
Source
Journal of the Arab American University
Issue
Vol. 6, Issue 1 (30 Jun. 2020), pp.1-14, 14 p.
Publisher
Arab American University Deanship of Scientific Research
Publication Date
2020-06-30
Country of Publication
Palestine (West Bank)
No. of Pages
14
Main Subjects
Abstract EN
-The peacock eye disease or the olive leaf spot caused by the fungus, Spilocae aoleagina, is the most olive destructive disease in many olive growing regions worldwide.
The disease causes yield losses of up to 20%.
Infection with the fungus depends on the environmental conditions and varies according to the different regions as well as olive genotype.
The aim of this work was to study the genetic variations between the isolates from different olive growing regions in Palestine.
In this work, six S.
oleagina isolates were collected from different regions in Palestine including Qalqilia, Nablus, Tulkarm, Ramallah, Jenin and Salfit.
Pure cultures of the fungus were obtained from single spore germination on PDA media.
PCR was used to identify the fungal isolates using species specific primers.
After using Box PCR, the diversity of the isolates was studied using the Box REPAIR primer.
BLASTn search of S.
oleagina isolates revealed similarities of 99% to S.
oleagina (Accession #.
AF338393.1).
Based on the analyses of the dendrogram obtained after Box PCR, two distinct isolates were obvious.
The first group (A) was divided into two subgroups (subgroup A1 included samples from Nablus and Ramallah and subgroup A2 included samples from Qalqilia, Salfit and Tulkarm).
The second group (B) included a sample from Jenin only.
To the best of the researchers' knowledge, this is the first work that identifies the genetic differences between S.
oleagina isolates in Palestine.
Further work is needed to examine the pathogenicity of the isolates on different olive genotypes.
American Psychological Association (APA)
Khulayf, Fatimah& Salman, Mazin. 2020. Genetic diversity among Spilocaea oleagina isolates from different regions in Palestine. Journal of the Arab American University،Vol. 6, no. 1, pp.1-14.
https://search.emarefa.net/detail/BIM-1333239
Modern Language Association (MLA)
Khulayf, Fatimah& Salman, Mazin. Genetic diversity among Spilocaea oleagina isolates from different regions in Palestine. Journal of the Arab American University Vol. 6, no. 1 (Jun. 2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1333239
American Medical Association (AMA)
Khulayf, Fatimah& Salman, Mazin. Genetic diversity among Spilocaea oleagina isolates from different regions in Palestine. Journal of the Arab American University. 2020. Vol. 6, no. 1, pp.1-14.
https://search.emarefa.net/detail/BIM-1333239
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2-13
Record ID
BIM-1333239