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Evaluation of antiviral activity of allium cepa and allium sativum extracts against newcastle disease virus
Joint Authors
Harazim, Rasha
Abd al-Rahman, Sahar
al-Qinawi, Ali
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 61, Issue 1 (30 Apr. 2019), pp.108-118, 11 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2019-04-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
-Newcastle Disease Virus (NDV) is the causative agent of the most devastating diseases affecting poultry especially in large scale production causing severe economic losses due to high mortality which may reach 100% in very virulent strains of NDV.
In the present study, NDV-suspected samples were isolated in 10 days-old Embryonated chicken Eggs (ECEs) via allantoic cavity for three passages.
Viral isolates were identified by Haemagglutination inhibition (HI) test, electron microscope and reverse transcriptase polymerase chain reaction (RT- PCR).
The antiviral effects of both onion and garlic extracts were studied on field isolates and reference strain of NDV.
The antiviral effects involve the gross lesions changes of the embryo in inoculated ECEs, Haemagglutination (HA) test and Infectivity test (EID50%).
The obtained results showed that most of the isolated viruses expressed subcutaneous Haemorrhage and death of the embryo at the fifth day post inoculation via allantoic cavity at the third passage of ECEs, while the field sample number (10) and the reference strain showed extensive haemorrhage and rapid death of embryos.
The isolated viruses were identified as NDV of the susceptible to be infected samples by HI using hyperimmune serum against NDV prepared in rabbit.
The Mean Death Time (MDT) of the field sample consisting of (10) and the reference strain which showed that the tested samples are velogenic strains was calculated.
Electron microscopy investigation of some selected samples (of high HA titers) identified the virus as pleomorphic.
Some selected samples were analyzed by RT- PCR and gel electrophoresis to express clear bands by using primers against the F-gene of NDV.
Brown leaves of onion (Allium Cepa) and bulbs of garlic (Allium sativum) were separately extracted.
These extracts were tested for their antiviral activity against the previously isolated and identified NDV.
Investigating the antiviral effects of both onion and garlic extracts on some field samples showed absence of virus activity after treatments by extracts, while samples number (10) and reference strain showed the reduction of virus in HA test, subcutaneous hemorrhage and EID50%.
The reduction of NDV infectivity may be due to the blocking of the attachment of the virus with the cell reports
American Psychological Association (APA)
Harazim, Rasha& Abd al-Rahman, Sahar& al-Qinawi, Ali. 2019. Evaluation of antiviral activity of allium cepa and allium sativum extracts against newcastle disease virus. Alexandria Journal of Veterinary Sciences،Vol. 61, no. 1, pp.108-118.
https://search.emarefa.net/detail/BIM-1333383
Modern Language Association (MLA)
Harazim, Rasha…[et al.]. Evaluation of antiviral activity of allium cepa and allium sativum extracts against newcastle disease virus. Alexandria Journal of Veterinary Sciences Vol. 61, no. 1 (Apr. 2019), pp.108-118.
https://search.emarefa.net/detail/BIM-1333383
American Medical Association (AMA)
Harazim, Rasha& Abd al-Rahman, Sahar& al-Qinawi, Ali. Evaluation of antiviral activity of allium cepa and allium sativum extracts against newcastle disease virus. Alexandria Journal of Veterinary Sciences. 2019. Vol. 61, no. 1, pp.108-118.
https://search.emarefa.net/detail/BIM-1333383
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-1333383