Tropical theileriosis, epidemiology and molecular diagnosis in cattle in new valley province, Egypt
Joint Authors
al-Nakir, Yasir F.
Mahir, Ahmad
Ahmad, Sutuhi
Abd al-Hakim, Usamah
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 63, Issue 1 (31 Oct. 2019), pp.31-41, 11 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2019-10-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
In this study during one year 294 blood samples, collected from cattle showing clinical signs arousing suspicion of theileriosis and apparently healthy neighboring animal (related one) for diagnosis of theileriosis using stained blood film and molecular diagnosis (Semi nested PCR).
the result of blood film revealed that 91 out of 294 examined cattle (30.95%) were positive for the piroplasmic form (intra erythrocytic stage) of T.
annulate infection, the highest prevalence of infection by theileriosis was (39.50 %) in Summer, and the lowest rate was (13.95 %) in Winter.
and the highest prevalence of infection was 39.30 %, in age group1 (1day – 6months) and the lowest rate was 21.40%.
in group 5 (> 4 Years).
the Tbs-S/Tbs-A primer set was used for PCR amplification of Theileria sp.
And the Ta-S/Tbs-A specific primer set was used in semi-nested PCR technique for detection of theileria annulata.
the semi-nested PCR accurately revealed (60%) positive samples the results indicated that giemsa staining method, having 50% sensitivity, is not suitable for determination of theileria annulata in carrier cattle, while semi-nested PCR technique can be used as a gold standard method for the mentioned aim and also can be used for screening of theileria annulata carrier cattle in all regions.
the phylogenic analysis of DNA of theileriosis revealed two sequences deposited in the GenBank database under accession numbers MK332380 and MK332382.
American Psychological Association (APA)
Ahmad, Sutuhi& Abd al-Hakim, Usamah& Mahir, Ahmad& al-Nakir, Yasir F.. 2019. Tropical theileriosis, epidemiology and molecular diagnosis in cattle in new valley province, Egypt. Alexandria Journal of Veterinary Sciences،Vol. 63, no. 1, pp.31-41.
https://search.emarefa.net/detail/BIM-1333527
Modern Language Association (MLA)
Ahmad, Sutuhi…[et al.]. Tropical theileriosis, epidemiology and molecular diagnosis in cattle in new valley province, Egypt. Alexandria Journal of Veterinary Sciences Vol. 63, no. 1 (Oct. 2019), pp.31-41.
https://search.emarefa.net/detail/BIM-1333527
American Medical Association (AMA)
Ahmad, Sutuhi& Abd al-Hakim, Usamah& Mahir, Ahmad& al-Nakir, Yasir F.. Tropical theileriosis, epidemiology and molecular diagnosis in cattle in new valley province, Egypt. Alexandria Journal of Veterinary Sciences. 2019. Vol. 63, no. 1, pp.31-41.
https://search.emarefa.net/detail/BIM-1333527
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 39-41
Record ID
BIM-1333527