Anti-inflammatory activity in vitro and in vivo of ethanolic extracts of stevia rebaudiana bertoni and trigonella foenum-graecum

Abstract EN

This study was based on evaluating anti-inflammatory activities of ethanolic extracts from Stevia leaves (EESL) and Fenugreek seeds (EEFS) (in vitro and in vivo).

In the in vitro experiment, the results exhibited potent anti-inflammatory activity.

The combination of both extracts (1: 1, 100 g) showed to be the most potent inhibitor of COX-2 with inhibition % (83.64% ).

This result was comparable with the standard Celecoxib showed higher level of inhibition, (86.63% , 100 g).

While, EESL showed the highest activity as COX-1 inhibitory activity (74.35% , 100 g), which it is higher than standard Celecoxib (67.64% , 100 g).

In vivo anti-inflammatory activity was performed on Wistar rats at the doses 200 and 400 mg/kg body weight using the carrageenan-induced rat paw edema modal.

Combination of (SLEE + FSEE, each 100 mg) displayed the highest significant anti-inflammatory activity (0.46ef± 0.025, p < 0.05) after 4 h, compared with indomethacin (0.44f ± 0.025, p < 0.05).

The results indicated that these plants may have a role for discovering new anti-inflammatory natural drugs.

Furthermore, Stevia leaves and Fenugreek seeds can be subjected to further investigations as anticancer agents.