A new trial in Egypt to detoxify AFM1 in UHT milk by lactobacilli and their bacteriocins

Abstract EN

Aflatoxins are potent carcinogenic, mutagenic and immune suppressive agents.

Acute exposures to aflatoxins cause rapid death due to liver failure.

Several strains of probiotics, frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media.

The purpose of this study was to evaluate the ability of heat killed Lactobacillus rhamnosus and Lactobacillus acidophilus and their bacteriocins to detoxify aflatoxin M1 (AFM1) in UHT (ultra-heat- treated) skim milk spiked with 5, 10, 20 ng/ml AFM1.Both strains were heat killed (100 oC /1hr) before binding in spiked skim milk, the unbound AFM1 was measured by HPLC.

Heat killed Lb.

acidophilus had a significantly higher capability to bind AFM1 in UHT skim milk with mean percentage of (0 %, 27.3±2.9 % and 39.07 ±10.33%) in comparsion with Lb.

rhamnosus (0%, 12.3±2.6% and 14.3± 3.48 %), respectively.

On other hand, bacteriocins of both strains showed a significantly AFM1 reduction ranged from 33 to 77%.

These findings shed light on the ability of heat killed Lb.

acidophilus, Lb.

rhamnosus and their bacteriocins to detoxify AFM1 in UHT skim milk.