Cryptosporidiosis : molecular analysis, risk factors and seasonal abundance in immunocompetent and immunocompromised patients, Kafrelsheikh university hospitals

Abstract EN

This study detected of the molecular cryptosporidium species in immunocompetent and imm-unocompromised adults with diarrhea attended Kafrelsheikh university hospitals and to assess risk factors.

stool samples from 200 immunocompetent & 200 immunocompromised were examined using acid fast (AF) stain and PCR / restriction fragment length polymorphism (RFLP) targeting small subunit (SSU) RRNA gene.

cryptosporidium PCR amplified was 15 (7.5%) in im-munocompetent and 23 (11.5%) immunocompromised, with significance difference as to age, presence of mucous, abdominal pain (P < 0.05).

cryptosporidium was more common in rural areas in autumn and winter seasons.

two genotypes were C.

hominis and C.

parvum, but C.

hominis was 60% in immunocompetent and 82.6% in immunocompromised.