Immunochromatography versus microscopy for diagnosis of entamoebia histolytica-dispar infection in Sohag University hospitals, Egypt

Abstract EN

Amoebiasis caused by E.histolytica protozoon, is a major public health worldwide.

delay in diagnosis and treatment may lead to fatality.

this study evaluated the efficacy of immunochromatographic test (ICT) using Rida® Quick Entamoeba test compared with traditional microscopic examination for E.histolytica / dispar infection.

a cross-sectional study was conducted on 100 patients suffering from dysentery or diarrhea with an age range of 3-62 years, attended Endemic diseases hospital, Sohag university hospitals.

stool samples were examined by direct wet smears, formalin ethyl acetate concentration methods, stained with Wiegert's iron hemat-oxylin and modified Ziehl-Neelsen stains.

microscopic examination showed 43 / 100 (43%) patients having E.histolytica / dispar.

Copr-oantigen detection in stool sample was done using Rida® Quick Entamoeba test.

the sensitivity, specificity, PPV & NPV of Rida® Quick Enta-moeba tests were 97.67%, 96.49%, 95.45% & 98.21% respectively with an accuracy of 97.7%, and without cross-reactivity with some endemic intestinal parasites.

the allover infection rate was significantly associated with residence (p < 0.036) and family size (p < 0.03), but neither associated with sex (p < 0.471) nor age (p < 0.344).

Rida® Quick Entamoeba test proved to be simple, rapid, convenient, sensitive and specific assay to diagnose E.histolytica / dispar infection for epidemiology.