In vitro antagonistic potential, plant growth-promoting activity and indole-3-acetic acid producing trait of bacterial isolates from spent mushroom substrate of Agaricus bisporus
Other Title(s)
التأثير المضاد، و النشاط المعزز لنمو النبات و ميزة إنتاج الأندول3-حمض الأسيتيك في مالمختبر من العزلات البكترية من السكاد المستهلك (كمبوست) بعد إنتاج فطر المشروم الدائري (Agaricus bisporus)
Joint Authors
al-Sadi, Abd Allah Bin Muhammad
al-Muammariyah, Shayma Nasir Hamid
Babu, Sathish
al-Mahmuli, Issa Hashil
Velazhahan, Rethinasamy
Source
Agricultural and Marine Sciences : Research Journal
Issue
Vol. 25, Issue 2 (31 Dec. 2020), pp.22-29, 8 p.
Publisher
Sultan Qaboos University College of Agricultural and Marine Sciences
Publication Date
2020-12-31
Country of Publication
Oman
No. of Pages
8
Main Subjects
Abstract EN
Spent mushroom substrate (SMS) is widely used as a fertilizer and to control plant diseases.
The microor- ganisms surviving in SMS play a crucial role in plant growth promotion and biocontrol activity.
In this study, an effort was made to isolate and characterize the bacterial species present in the SMS of Agaricus bisporus and to study their antagonistic potential, plant growth-promoting ability and indole-3-acetic acid (IAA) producing trait.
Six different bacterial isolates exhibiting morphological variabilities were obtained from the SMS by serial dilution technique.
On the basis of 16S rRNA gene sequences, these isolates were identified as Staphylococcus epidermidis (Sh1 and Sh3), S.
aureus (Sh2), Bacillus albus (Sh4), Delftia lacustris (Sh6) and Comamonas aquatica (Sh7).
These bacterial strains were assayed for their antagonism against Pythium aphanidermatum, a phytopathogenic oomycete.
The results of in vitro dual culture assay revealed that all the 6 bacterial isolates showed low levels of suppression of P.
aphanidermatum and recorded less than 5 mm inhibition zone.
Among the bacterial isolates, S.
epidermidis Sh3 recorded the maximum inhibition zone of 4.2 ± 0.5 mm.
Plant growth promotion test using roll paper towel method revealed that C.
aquatica Sh7, B.
albus Sh4, D.
lacustris Sh6 and S.
epidermidis Sh3 caused a significant increase in seedling vigour of cucumber compared to control.
The seeds treated with the bacterial isolate C.
aquatica Sh7 showed the maximum seedling vigor (2018 ± 255).
Assessment of in vitro production of IAA by the bacterial isolates revealed that the bacterial isolates highly varied (ranging from 0.28 to 9.25 mg L-1) in their potential for production of IAA.
The maximum amount of IAA was produced by C.
aquatica Sh7 (9.25 ± 0.02 mg L-1).
Further studies are required to assess the possibility of using the IAA-producing bacterial isolates identified in this study or their metabolites to promote plant growth or to enhance growth and yield of mushrooms.
American Psychological Association (APA)
al-Muammariyah, Shayma Nasir Hamid& al-Sadi, Abd Allah Bin Muhammad& Babu, Sathish& al-Mahmuli, Issa Hashil& Velazhahan, Rethinasamy. 2020. In vitro antagonistic potential, plant growth-promoting activity and indole-3-acetic acid producing trait of bacterial isolates from spent mushroom substrate of Agaricus bisporus. Agricultural and Marine Sciences : Research Journal،Vol. 25, no. 2, pp.22-29.
https://search.emarefa.net/detail/BIM-1341383
Modern Language Association (MLA)
al-Sadi, Abd Allah Bin Muhammad…[et al.]. In vitro antagonistic potential, plant growth-promoting activity and indole-3-acetic acid producing trait of bacterial isolates from spent mushroom substrate of Agaricus bisporus. Agricultural and Marine Sciences : Research Journal Vol. 25, no. 2 (2020), pp.22-29.
https://search.emarefa.net/detail/BIM-1341383
American Medical Association (AMA)
al-Muammariyah, Shayma Nasir Hamid& al-Sadi, Abd Allah Bin Muhammad& Babu, Sathish& al-Mahmuli, Issa Hashil& Velazhahan, Rethinasamy. In vitro antagonistic potential, plant growth-promoting activity and indole-3-acetic acid producing trait of bacterial isolates from spent mushroom substrate of Agaricus bisporus. Agricultural and Marine Sciences : Research Journal. 2020. Vol. 25, no. 2, pp.22-29.
https://search.emarefa.net/detail/BIM-1341383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 28-29
Record ID
BIM-1341383