Isolation of salmonella characterized by biofilm formation and disinfectant resistance from broiler chickens
Joint Authors
Nabil, Nahhal M.
Yunus, Ahlam E.
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 62, Issue 2 (31 Jul. 2019), pp.26-36, 11 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2019-07-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study was carried out to isolate Salmonella from different broiler farms in Delta, Egypt and to investigate their antimicrobial susceptibility pattern with a molecular detection of genes responsible for biofilm formation and resistance to disinfectants.
Five Egyptian Governorates in Delta (Dakahila, Damietta, Gharbia, Kafr El Sheik and Sharkia) during the year 2018 were included in this study.
A total of 100 diseased farms were subjected for clinical followed by laboratory diagnosis.
Clinical diagnosis was made on the basis of clinical history, signs and postmortem findings of the affected broiler chickens.
Internal organs (liver, cecum, spleen and heart) and swabs form water pipes, waterers and feeders were collected.
Salmonella was isolated with an incidence rate of (17%).
The incidences of Salmonella isolation from the collected samples in Dakahila, Damietta, Gharbia, Kafr El Sheik and Sharkia Governorates were (5/29) (17.24%), (4/23) (17.39%), (3/20) (15%), (2/13) (15.38%) and (3/15) (20%) respectively.
The isolated Salmonellae were identified serologically and six serotypes were reported (S.
Typhimurium, S.
Kentucky, S.
Inganda, S.
Infantis, S.
Newport and S.
Enteritidis).
Antimicrobial sensitivity testing revealed that ampicillin – sulbactam, tetracycline and streptomycin showed higher resistance with percentages (94.1%), (94.1%) and (70.6%) respectively.
Using conventional PCR technique; AdrA, gcpA and csgD genes that responsible for biofilm formation were reported in all Salmonella isolates.
qacED1 gene was detected with percentages of (100%) but qacA/B and qacC/D genes were negative.
American Psychological Association (APA)
Nabil, Nahhal M.& Yunus, Ahlam E.. 2019. Isolation of salmonella characterized by biofilm formation and disinfectant resistance from broiler chickens. Alexandria Journal of Veterinary Sciences،Vol. 62, no. 2, pp.26-36.
https://search.emarefa.net/detail/BIM-1341425
Modern Language Association (MLA)
Nabil, Nahhal M.& Yunus, Ahlam E.. Isolation of salmonella characterized by biofilm formation and disinfectant resistance from broiler chickens. Alexandria Journal of Veterinary Sciences Vol. 62, no. 2 (Jul. 2019), pp.26-36.
https://search.emarefa.net/detail/BIM-1341425
American Medical Association (AMA)
Nabil, Nahhal M.& Yunus, Ahlam E.. Isolation of salmonella characterized by biofilm formation and disinfectant resistance from broiler chickens. Alexandria Journal of Veterinary Sciences. 2019. Vol. 62, no. 2, pp.26-36.
https://search.emarefa.net/detail/BIM-1341425
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 34-36
Record ID
BIM-1341425