Effects of melatonin and allium sativum (garlic)‎ on dibutyl phthalate induced oxidative stress on serum hormones and lipid profile of rabbit bucks

Abstract EN

Forty two rabbit bucks were used for this study, bucks were randomly divided into 7 groups of 6 each.

Group A was administered olive oil orally for 16 weeks, group B (olive oil + dibutyl phthalate (DBP) for 16 weeks), group C (melatonin for 8 weeks, then olive oil + DBP for another 8 weeks), group D (garlic for 8 weeks, then olive oil + DBP for another 8 weeks), group E (olive oil + DBP for 8 weeks, then melatonin for another 8 week), group F (olive oil + DBP for 8 weeks, then garlic for another 8 weeks) and group G (olive oil + DBP for 8 weeks, then melatonin and garlic for another 8 weeks).

The study lasted for 120 days, during which blood samples were collected on week 8 (to assess the pretreatment effects of melatonin and garlic, also induction of oxidative stress with DBP) and week 16 (to assess the protective and ameliorating effects of melatonin and garlic) in serum hormonal profile determination and on the 120th day of the study, 3mls of blood was collected during sacrifice of each buck into a non EDTA sample container for serum lipid profile determination and ejaculates was collected before sacrifice for semen parameters.

Results show significant differences (P < 0.0001) in mean FSH, LH and testosterone (T) concentrations between DBP exposed and treatment groups.

The results of serum lipid profile showed significant difference (P = 0.0086), in the mean serum total cholesterol concentration (mmol/L) between group B (4.08 ± 0.09) and groups (C, 2.5 ± 0.39; D, 2.35 ± 0.22; E, 3.2 ± 0.50; G, 2.9 ± 0.29), also there was significant difference (P = 0.0066) in the mean serum triglycerides concentration (mmol/L) between group B (1.83 ± 0.21) and all other groups (A, 0.88 ± 0.14; C, 1.15 ± 0.10; D, 1.28 ± 0.17; E, 1.1 ± 0.12; F, 1.35 ± 0.06 and G, 1.2 ± 0.15).

There was significant difference in semen parameters between group B and other groups of the study.

Conclusion DBP decreases the concentration of serum hormones and increases total cholesterol and triglycerides.