Flow cytometry analysis of dog bone marrow stem cell surface protein expression

Abstract EN

The present work aimed to have a look at the Flow cytometry analysis of dog bone marrow stem cellular floor protein expression.

Five healthful mongrel dogs (3 male dogs and 2 female dogs).

The dogs weight was (24 -50 kg b.Wt) and common age was (8 -24 Months), the dogs examined towards diseases.

The dogs were absolutely sterilized against any outside parasites or any outside diseases.Flow cytometry analysis of the surface markers against the selected phenotypes following cell surface protein expression (CD44, CD29, CD73, CD90, CD105, CD166, CD271, CD45, CD34, CD13, and c-kit (all from BD Biosciences).

BM-MSC at third passage were cultured in DMEM (Dulbecco’s Modified Eagle Medium) deprived of FCS (fetal calf serum), and supernatants were collected after 6 or 24 hours microvesicles.

MVs purification was performed using the ExoQuick-TC exosome precipitation solution (ExoQuick; System Biosiences) and storage at −80°C until further use.

It can be concluded that, the canine BM-MSCs play an vital role in veterinary medication and can assist in treating many sicknesses and harm that the animals going through it, flow cytometery can help for evaluation and identity of this cells, so it is able to be used easily for treating positive sicknesses and injuries.

Also, our results offer fundamental records to allow for more reproducible and eliable nice manage in the identity of canine BM-MSCs.