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Monitoring of variable stages of ovarian cells in buffalo using double stained SDS-PAGE
Author
al-Shuhaib, Muhammad Baqir Sahib Ahmad
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 24, Issue 3 (30 Sep. 2016), pp.671-676, 6 p.
Publisher
Publication Date
2016-09-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
This study focused on applying gel electrophoresis parameter as primary indicator to estimate and to pursue the approximate growth stage of the variable ovarian samples of buffalos.
several buffalo ovaries were taken from the local slaughter houses.
different positions of ovarian cells were isolated from each ovary.
samples were subjected to electrophoresis on denaturing polyacrylamide gel.
optimizations were taken place in matter of proteins concentration and staining techniques.
several ovarian bands were resolved and the monitoring of the resulting protein profile was taken place.
the developmental stages of ovarian cells were highlighted electrophoretically.
additionally, comparative electrophoresis was performed between the SDS-PAGE profile of amniotic fluid of buffalo and its corresponding bovine fluids.
concerning buffalo oocytes electrophoretic profile, significant differences were obtained among variable stages of development.
on the other hand, it's possible to use electrophoresis to compare between buffalo follicular fluid and its corresponding bovine counterpart.
American Psychological Association (APA)
al-Shuhaib, Muhammad Baqir Sahib Ahmad. 2016. Monitoring of variable stages of ovarian cells in buffalo using double stained SDS-PAGE. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 24, no. 3, pp.671-676.
https://search.emarefa.net/detail/BIM-1347732
Modern Language Association (MLA)
al-Shuhaib, Muhammad Baqir Sahib Ahmad. Monitoring of variable stages of ovarian cells in buffalo using double stained SDS-PAGE. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 24, no. 3 (2016), pp.671-676.
https://search.emarefa.net/detail/BIM-1347732
American Medical Association (AMA)
al-Shuhaib, Muhammad Baqir Sahib Ahmad. Monitoring of variable stages of ovarian cells in buffalo using double stained SDS-PAGE. Journal of Babylon University : Journal of Applied and Pure Sciences. 2016. Vol. 24, no. 3, pp.671-676.
https://search.emarefa.net/detail/BIM-1347732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 675-676
Record ID
BIM-1347732