Immunostaining method to explore cellular immunity against toxoplasma Gondii in Iraqi women with a history of abortion

Abstract EN

Otjcdioc : To explore cellular immunity against Toxoplasma gondii in Iraqi women with a history of abortion using the immunostaining method.

/Hethohs : Sixty women who had abortion were selected for this study.

The presence of anti-Toxoplasma antibodies in their sera was confirmed via ELISA testing.

Heparinized blood and serum were collected.

The women were divided into three groups according to the presence or absence of specific anti-Toxoplasma antibodies in their serum- These groups included : women with IgG (18), women with IgM (14) and women with both IgG and IgM (12).

The other 16 women had no antibodies against Toxoplasma.

Twenty-four healthy-looking women had been selected as controls.

Those that revealed any antibody titer against Toxoplasma were excluded from the study.

The isolated peripheral blood leukocytes (PEL) were tested for cell surface markers (CD3, CD4, CDs and CD71) using monoclonal antibodies against T-cell subsets.

Results : All the surface markers revealed significantly higher percentages when compared to the healthy controls.

Conclusion : The results indicate a strong cellular immune response during toxoplasmosis.