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Genetic similarity of two different color local guinea fowl (numida meleagris) by using rapd-pcr technique
Author
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 32, Issue 2 (30 Apr. 2020), pp.140-144, 5 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2020-04-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The aim of present study to determine DNA polymorphism, between and within two different color (white) and (black) guinea fowl lines.
Blood samples were collected about 3 ml of 30 white guinea fowl and 30 black guinea fowl, in six different local farms.
DNA was isolated, the purity of the DNA samples ranged from 1.8 to 1.9.
RAPD – PCR was used to evaluate genetic similarity between the lines of guinea fowl.
Using eighteen primers for amplification of RAPD, from Gen Script USA.
A total of (14) primers out of (18) primers gave results to find Genomic DNA complementary sites.
After electrophoresis the largest number of bands were appeared with OPA-03 primers, but the smallest was obtained when OPQ-15 primer used.
Determined Genetic similarity as the mean for the primers used, between the black and white guinea fowl, was 0.76.
The coefficient of genetic similarity within white guinea fowl line, was 0.78 and 0.73 within black guinea fowl line.
American Psychological Association (APA)
Abd al-Razzaq, Hurea S.. 2020. Genetic similarity of two different color local guinea fowl (numida meleagris) by using rapd-pcr technique. ZANCO Journal of Pure and Applied Sciences،Vol. 32, no. 2, pp.140-144.
https://search.emarefa.net/detail/BIM-1386561
Modern Language Association (MLA)
Abd al-Razzaq, Hurea S.. Genetic similarity of two different color local guinea fowl (numida meleagris) by using rapd-pcr technique. ZANCO Journal of Pure and Applied Sciences Vol. 32, no. 2 (2020), pp.140-144.
https://search.emarefa.net/detail/BIM-1386561
American Medical Association (AMA)
Abd al-Razzaq, Hurea S.. Genetic similarity of two different color local guinea fowl (numida meleagris) by using rapd-pcr technique. ZANCO Journal of Pure and Applied Sciences. 2020. Vol. 32, no. 2, pp.140-144.
https://search.emarefa.net/detail/BIM-1386561
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 144
Record ID
BIM-1386561