Evaluating the prevalence of virulence factor gene and biofilm production in Pseudomonas aeruginosa isolated from different clinical samples

Abstract EN

Pseudomonas aeruginosa is considered a resourceful pathogen; which has several essential virulence effectors such as exoenzyme, exotoxin and biofilm might help to it is infection.

This study aimed to investigate the frequency of exoS gene, the determination of biofilm production and antimicrobial resistance among clinical samples of P.

aeruginosa.

In our study, 227 specimens of P.

aeruginosa collected from different clinical specimens which were attending public hospitals in Erbil city.

Antimicrobial resistance of samples identified by Kirby-Bauer disk diffusion method.

Through PCR virulence gene exoS was studied.

Biofilm production measured by both Congo Red Agar (CRA) and tissue culture plate method (TCP).

Among 227 clinical samples, 40 (17.6%) were positive for P.

aeruginosa.

Imipenem was showed most effective antibiotic 95% against P.

aeruginosa.

Incidence of exoS gene was 70% within the P.

aeruginosa isolates.

Moreover, around 75% of clinical samples produced biofilm and approximately 40% of them produced strong biofilm.

Our study showed that the incidence of bla exoS gene and biofilm formation, which are common virulence factors in the clinical samples, especially in burn patients, and are a severe problem in the treatment of the patient.