Phytochemical screening and anti-candida activities of Crocus cancellatus herb. Ethanol extract

Abstract EN

The antifungal, antioxidant activity, total tannin estimation, and phytochemical screening of Crocus Cancellatus extract were investigated.

The highest yield was obtained with ethanol solvent (36.1%).

A significant amount of tannin was obtained in the plant (11.1%).

In Antifungal testing against Candida spp.

The most effective Inhibitory obtained was 13.16mm against C.

Krusei, while the less effective activity was 8.22 mm against C.

Glabrata.

The minimum inhibitory concentration (MIC) value obtained against all tested Candida Spp.

candida strains within diameter rate of 12.5-25 μg/ml.

The minimum inhibitory Effects of tested antifungal agents was FLU/25 which have no any inhibitory effects especially against was Candida famata While the highest inhibitory zone of FLU/25 38.40 mm was obtained against Candida guilliermondii.

the maximum antioxidant activity obtained was 98.68% at 0.2 ml.

10 compounds were determined in the content determination study with LC-MS/MS.

Gallic acid (46965.14 μg/g), Quinic acid (1935.71 μg/g), and malic acid (3831.37μg/g) were obtained at the highest levels.

Vanillic acid (138.2 μg/g), Protocatechuic acid (116.87μg/g), tr-Ferulic acid (51.17μg/g), Quercetin (20.56 μg/g), p-coumaric acid (12.8 μg/g), Chlorogenic acid (2.51 μg/g) and Salicylic acid (1.56 μg/g) were obtained quantitatively less.

These results confirmed that the Crocus cancellatus ethanol extract has potential antioxidant and antifungal activity.