Impact of vitamin D3 nanoemulsion on spermatogenesis and antioxidant enzymes in Vitamin D deficient induced albino male rats
Joint Authors
Karim, Diyar Hamid
Aziz, Hoshyar Abd Allah
Muhammad, Sulaf Mustafa
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 33, Issue 1 (28 Feb. 2021), pp.55-67, 13 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2021-02-28
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
Vitamin D deficiency is common with several effects that include nonskeletal impacts.
Many studies showed associations between Vitamin D deficiency and oxidative statues with disturbed testicles in male rats (Rattus norvegicus) which might be improved by vitamin D.
The aim of this work is to find the effects of nanoemulsion pea protein isolates and novel vitamin D carrier, developed by sonication and pH shifting of pea protein isolate nanoemulsion on the spermatogenesis in induced vitamin D deficient male rats.
Thirty male albino rats distributed into 5 groups, sufficient control was fed with normal diet, deficient control was treated with 100 mg/kg Rimfapicin and 50 mg/kg Isoniazed for three weeks, pea protein isolate group :vitamin D deficient rats treated daily with 60 mg/ml/kg nanoemulsion pea protean isolate, Vitamin D deficient rats were treated with vitamin D (54 mcg/ml/ kg), and pea protean isolate + vitamin D group: Vitamin D deficient rats were treated by nanoemulsion pea protein isolate (60 mg/dl/kg) plus Vitamin D (54 mcg/ml/ kg) for four weeks.
The serum used for Vitamin D and total testosterone assay, after estimation of sperm count and morphology.
Homogenized testes used for determination of Catalase and Glutathione peroxidase.
The results revealed that vitamin D and supplementations each one alone have a positive significant effects on spermatogenesis.
The nanoemulsion pea protein isolate +vitamin D resulted in a significant increase in the levels of vitamin D of total testosterone more than the supplementation of vitamin D and nanoemulsion pea protein isolate alone.
Also sperm count, normal sperm morphology, as well as catalase and Glutathione peroxidase improved significantly in this group.
In conclusion we demonstrated vitamin D nanoemulsion as more efficient formulation with more prominent effects on spermatogenesis in induced vitamin D deficient rats.
American Psychological Association (APA)
Karim, Diyar Hamid& Muhammad, Sulaf Mustafa& Aziz, Hoshyar Abd Allah. 2021. Impact of vitamin D3 nanoemulsion on spermatogenesis and antioxidant enzymes in Vitamin D deficient induced albino male rats. ZANCO Journal of Pure and Applied Sciences،Vol. 33, no. 1, pp.55-67.
https://search.emarefa.net/detail/BIM-1387807
Modern Language Association (MLA)
Karim, Diyar Hamid…[et al.]. Impact of vitamin D3 nanoemulsion on spermatogenesis and antioxidant enzymes in Vitamin D deficient induced albino male rats. ZANCO Journal of Pure and Applied Sciences Vol. 33, no. 1 (2021), pp.55-67.
https://search.emarefa.net/detail/BIM-1387807
American Medical Association (AMA)
Karim, Diyar Hamid& Muhammad, Sulaf Mustafa& Aziz, Hoshyar Abd Allah. Impact of vitamin D3 nanoemulsion on spermatogenesis and antioxidant enzymes in Vitamin D deficient induced albino male rats. ZANCO Journal of Pure and Applied Sciences. 2021. Vol. 33, no. 1, pp.55-67.
https://search.emarefa.net/detail/BIM-1387807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 64-67
Record ID
BIM-1387807