Estimation of (TaGSK1)‎ gene expression related with salt tolerance in four bread wheat cultivars (Triticum aestivum L.)‎

Joint Authors

Ismail, Iman N.
Abid, Ashwaq S.
Majid, Duha M.
al-Jabburi, Abd al-Jasim M. Jasim

Source

ZANCO Journal of Pure and Applied Sciences

Publisher

Salahaddin University-Erbil Department of Scientific Publications

Publication Date

2017-08-31

Country of Publication

Iraq

No. of Pages

6

Main Subjects

Biology

English Abstract

Salinity stress and low water availability affect crop plants in agricultural systems, limiting the productivity of different crops because the mineral toxicity with deficiency frequently causing high osmotic stress as well as overlap toxic ions with main metabolic processes.

The aim of this work was to study (TaGSK1) gene expression in some selected cultivars of bread wheat (Furat and Dijila) which selected for salt tolerance through plant breeding programs, while (Lateffyia and Tamooze-2) are Iraqi local cultivars, using real-time PCR TaqMan gene expression assays.

(TaGSK1) gene expression was detected by agarose gel electrophoresis in Furat and Dijila cultivars with amplicon products sizes (189 bp) product while it was absent in (Lateffyia and Tamooze-2) cultivars under both of salinity and non-salinity stress.

Real time PCR quantification showed that rising of salt stress levels led to increasing the gene expression, so the highest expression values of (TaGSK1) gene were (8.491 x 1015 and 8.379 x 1015) respectively in Furat and Dijila cultivars at high salt stress level (20 ds/m).

Data Type

Conference Papers

Record ID

BIM-1388805

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