Determination of metronidazole in pharmaceutical products based on the formation of azo dye

Joint Authors

Fakhr, Nabil A.
Kokhasmail, Dashne M.

Source

ZANCO Journal of Pure and Applied Sciences

Publisher

Salahaddin University-Erbil Department of Scientific Publications

Publication Date

2017-08-31

Country of Publication

Iraq

No. of Pages

9

Main Subjects

Chemistry
Pharmacology

English Abstract

Two simple, rapid, sensitive and accurate spectrophotometric methods have been developed for the estimation of metronidazole.

The method depends on the reduction of Metronidazole molecule with zinc powder and hydrochloric acid followed by diazotization and coupling with 2-hydroxybenzaldehyde to give yellow colored in alkaline medium with an absorption maximum at 457 nm.

Under optimized experimental conditions Beer's law was obeyed in the concentration ranges 3.0 to 15.0 μg/ml and 25.0 to 37.0 μg/ml.

Also, by using p-aminoacetophenone as a coupling agent to form orange colored with an absorption maximum at 490 nm.

Beer's law was obeyed in the concentration ranges 3.0 to 40.0 μg/ml.

The Sandell's sensitivity, the molar absorptivity, correlation coefficient, detection limit and the regression equation were calculated.

Both techniques were applied successfully to a wide variety of pharmaceutical preparations

Data Type

Conference Papers

Record ID

BIM-1388955

• APA
• MLA
• AMA