Identification of some drought and salinity genes in four wheat cultivars (triticum aestivum)
Joint Authors
Rafat, Muhammad H.
Salim, Tamir M.
Ahmad, Islam Mahmud
Bikhit, Makhluf M.
Source
Annals of Agricultural Science, Moshtohor
Issue
Vol. 60, Issue 1 (31 Mar. 2022), pp.113-117, 5 p.
Publisher
Banha University Faculty of Agriculture
Publication Date
2022-03-31
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Topics
Abstract EN
Four wheat cultivars, Gemmeiza10, Misr1, Sakha93, and Giza168 were used in this study as well as the hybrids resulted between all the studied four cultivars.
Misr1 Sakha93 resulted hybrids were identified using salinity primers Na+/H+ antiporter gene amplification followed by sequencing while Giza168 Gemmeiza10 resulted hybrids were identified by drought primers DREB2 gene amplification followed by sequencing.
The resulted sequences were compared with those available on NCBI website database through the BLAST bioinformatics tool.
The obtained data showed that, the base size of Na+/H+ antiporter gene was 400 bp while the base size of DREB2 gene was 200 bp.
the phylogenetic analysis showed that Na+/H+ antiporter gene obtained sequence was related to four accession numbers from gene bank.
In addition, phylogenetic analysis exhibited an amount of genetic change on the root of clades 0.0020, where the Na+/H+ antiporter gene -based phylogeny tree included four clades.
The clades grouping had low support (bootstrap value between 6-8% ).
Also, the obtained DREB2 gene sequences were aligned with six accession numbers in the NCBI website database as shown from the phylogeny tree.
The phylogenetic tree deducted from the sequence comparison of DREB2 gene region showed that the length of branch that represents an amount of genetic change of 0.0020, where it's based phylogeny tree included four clades.
The clades grouping had low support (bootstrap value between 8-12% ).
American Psychological Association (APA)
Ahmad, Islam Mahmud& Rafat, Muhammad H.& Bikhit, Makhluf M.& Salim, Tamir M.. 2022. Identification of some drought and salinity genes in four wheat cultivars (triticum aestivum). Annals of Agricultural Science, Moshtohor،Vol. 60, no. 1, pp.113-117.
https://search.emarefa.net/detail/BIM-1390676
Modern Language Association (MLA)
Rafat, Muhammad H.…[et al.]. Identification of some drought and salinity genes in four wheat cultivars (triticum aestivum). Annals of Agricultural Science, Moshtohor Vol. 60, no. 1 (2022), pp.113-117.
https://search.emarefa.net/detail/BIM-1390676
American Medical Association (AMA)
Ahmad, Islam Mahmud& Rafat, Muhammad H.& Bikhit, Makhluf M.& Salim, Tamir M.. Identification of some drought and salinity genes in four wheat cultivars (triticum aestivum). Annals of Agricultural Science, Moshtohor. 2022. Vol. 60, no. 1, pp.113-117.
https://search.emarefa.net/detail/BIM-1390676
Data Type
Journal Articles
Language
English
Notes
-
Record ID
BIM-1390676